Biological Monitoring

Supervised Urine Collections – Protocol

Approved: June 25, 2008
Revised: June, 2014

A supervised screen is conducted in order to ensure that an unadulterated specimen is obtained, correctly identified, not diluted or in any way, adulterated or substituted during the collection process. Identification on the bottle and requisition form sent to the testing laboratory will identify the individual by their program code number.

A record of the specimen collection, including date, time and the name of the supervisor will be maintained in a secure area.

Collection Procedure:

  1. The donor, after washing hands, will remain in the presence of the supervisor and will not have access to water from a tap, soap dispenser, cleaning agent or any other materials that may adulterate a specimen.
  2. The supervisor and the donor will proceed to a predetermined washroom that will accommodate two people.
  3. The supervisor will directly watch over the collection of the specimen.
  4. When handed the bottle, the supervisor will inspect the urine specimen to determine its color and look for any indication of adulterants of diluents. The temperature will be recorded and should be in the range of 33oc to 38oc. Any unusual finding will be noted in the supervisor’s records.
  5. If the validity of the specimen is suspect, the donor will be asked to remain on site and provide another sample as soon as possible.  Both specimens will be forwarded to the laboratory for testing.
  6. Both the supervisor and the donor will keep the specimen in full view at all times prior to it being sealed and labeled.
  7. The supervisor will request that the donor observe the “Confidential Seal” being filled out and being placed on the vial and shall sign the seal.
  8. The supervisor, with the donor observing, will fill out the “Chain of Custody Requisition” and the donor will be asked to sign off the information on the form.
  9. After the supervisor has placed the sealed, labeled vial into the plastic bag, the donor will sign across the seal of the bag.
  10. Only when the specimen has been provided and submitted to the supervisor will the donor be allowed to wash their hands.
  11. If the specimen is not able to be transported immediately to the laboratory, the specimen, in the sealed bag, will be kept refrigerated in a secure location.


Dr. Jeremy Beach, Assistant Registrar
780-969-4940 or 1-800-561-3899 ext. 4940 (in Canada)

Leanne Minckler, Physician Health Advisor
780-969-4943 or 1-800-561-3899 ext. 4943 (in Canada)